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. 2024 Feb 27;12(2):e1199. doi: 10.1002/iid3.1199

Figure 3.

Figure 3

KLF4 positive regulation of TXNIP transcription expression. (A) Using UCSC online promoter analysis software to predict the promoter region of TXNIP gene and the transcription factor database JASPAR for transcription factors that may interact with the promoter region of TXNIP. We found that there are five KLF4 binding sites in the promoter region of TXNIP. (B) Schematic diagram of binding site primer design. (C) Luciferase report confirms that KLF4 regulates TXNIP transcription in lipopolysaccharide/adenosine 5‐triphosphate‐induced intestinal pyroptosis model. (D) Y2H screening for interactions between KLF4 and TXNIP. We have constructed plasmids for KLF4‐AD and TXNIP‐BD, respectively, yeast can only survive on the SD‐Leu‐Trp‐ His medium when the AD and BD of the two plasmids interact, otherwise they cannot survive. (E) CO‐IP assay showed that there is a direct interaction between KLF4 and TXNIP. *p < .5; **p < .01.