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| Bacteria | Medium | Incubation time | Conditions |
|---|---|---|---|
| Aac | THB + 10% yeast extract | 48 h | 37 °C, 5% CO2 |
| A. naeslundii | BHI + 10 μg mL−1 vitamin K | 24 h | 37 °C, anaerobe |
| P. gingivalis | FAB | 96 h | 37 °C, anaerobe |
| S. oralis | BHI + 10 μg mL−1 vitamin K | 24 h | 37 °C, anaerobe |
