Figure 1.

Experimental design and NAc cell cluster identification. a, Treatment paradigms and sample sizes for acute or repeated cocaine experiments. Rats (N=16/protocol, 8M/8F) received intraperitoneal injections of saline or cocaine (20mg/kg), and brain tissue was harvested 1 h after injections. NAc tissue was used for microfluidics-based nuclei capture, barcoding, and snRNA-seq on the 10X Genomics platform. The acute cocaine dataset was previously published in Savell, Tuscher, Zipperly, Duke, Phillips, et al., 2020 Science Advances. Repeated and acute cocaine datasets were integrated for further analysis. b, UMAP displaying 16 transcriptionally distinct cell types identified in the NAc from the integrated dataset (n=39,254 nuclei). c, UMAPs showing distribution of cells from acute and repeated cocaine experiments across all cell types. d, Violin plots displaying expression levels of marker genes of each cell type. e, Feature plots showing distribution of expression values across all cell types for marker genes for all neurons (Syt1), GABAergic cells (Gad1), medium spiny neurons (Bcl11b), oligodendrocytes (Mbp), microglia (Arhgap15), and astrocytes (Gja1).