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. Author manuscript; available in PMC: 2024 Jun 1.
Published in final edited form as: Mol Cell Neurosci. 2023 Mar 24;125:103849. doi: 10.1016/j.mcn.2023.103849

Figure 2.

Figure 2.

Identification of genes and pathways enriched in transcriptionally distinct populations of Drd1-expressing MSNs. a, UMAP highlighting the two identified populations of Drd1-expressing MSNs. b, Results of pseudobulk analysis directly comparing cells within the Drd1-MSN-1 population to cells within the Drd1-MSN-2 population. Enriched genes are those with an adjusted p-value < 0.05 and a ∣log2(Fold Change)∣ > 0.5. 506 genes were found to be enriched in the Drd1-MSN-1 population and are highlighted in red with a positive log2(Fold Change). 665 genes were found to be enriched in the Drd1-MSN-2 population and are highlighted in blue with a negative log2(Fold Change). c, Molecular function gene ontology analysis highlights enrichment of calcium-dependent processes in Drd1-MSN-1 population, while potassium-dependent processes are enriched in the Drd1-MSN-2 population. d, Dot plot showing expression levels and percent of cells within the clusters expressing genes involved in calcium- or potassium-dependent processes. e-f, Scatter plots highlighting differentially expressed genes with high counts and high Gini coefficients. This analysis identified Ebf1 and Htr4 as potential markers of Drd1-MSN-1 and Drd1-MSN-2, respectively. g, Feature plots showing enrichment of Bcl11b (MSN marker), Drd1 (Drd1-MSN marker), Ebf1 (Drd1-MSN-1 marker), and Htr4 (Drd1-MSN-2 marker).