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. Author manuscript; available in PMC: 2024 Jun 1.
Published in final edited form as: Mol Cell Neurosci. 2023 Mar 24;125:103849. doi: 10.1016/j.mcn.2023.103849

Figure 3.

Figure 3.

In situ validation of transcriptionally distinct populations of Drd1-expressing MSNs. a, Representative 20x stitched image following RNAscope in situ hybridization with probes for Drd1, Ebf1, Htr4, and DAPI. Scale bar: 1mm. ac: anterior commissure; cc: corpus callosum; lv: lateral ventricle; vp: ventral pallidum. ICjM: Islands of Calleja Major, b, 100x images showing representative Drd1+/Ebf1−/Htr4−, Drd1+/Ebf1+/Htr4−, Drd1+/Ebf1−/Htr4+, and Drd1+/Ebf1+/Htr4+ cells, with Drd1, Ebf1, Htr4, and merged channels. Scale bar: 10μm. c, Quantification and comparison of Drd1+ subpopulations in the NAc. One-way ANOVA test with Tukey’s multiple comparison test, d, Location of cells expressing selected genes and anatomical overlay, e, Quantification and comparison of Drd1+ subpopulations in the NAc core and shell. One-way ANOVA test with Tukey’s multiple comparison test, f, Proportion of Drd1+ cell types in the NAc from RNAscope (n=6 animals) and snRNAseq datasets. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001, ns: not significant.