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. 2024 Feb 5;20(2):e1011152. doi: 10.1371/journal.pgen.1011152

Fig 2. Expression and subcellular localization of Rab4, Rab5, Rab7 and Rab11 in vitellogenic oocytes.

Fig 2

Confocal images of stage 10 egg chambers double-labeled for endogenous Rab7 (red) and eGFP or YFP tag (green) for (A) Yl-eGFP-3xHA, (B) YFP-Rab5, (C) YFP-Rab11 and (D) YFP-Rab4, respectively, from the corresponding endogenous tagging fly lines. Yolk granules within the same oocytes were visualized by DIC (gray). (A-D) Overview of Rab7 (A), YFP-Rab5 (B), YFP-Rab11 (C) and YFP-Rab4 (D) in the imaged oocytes, respectively. (A1-D4) High-magnification view of the corresponding cortex regions highlighted in (A-D), respectively, as indicated. Endogenous Rab7 used as reference for endosomes in all the images. Images were shown in individual channels in gray or as overlaying images in color, as indicated. Genotypes: (A) w1118; p{w(+mC), yl-eGFP-3xHA}. (B) w1118; TI{TI} EYFP-Rab5 /CyO (BDSC #62543). (C) w1118; TI{TI} EYFP-Rab11 (BDSC #62549). (D). y1, w1118; TI{TI} EYFP-Rab4 (BDSC #62542). The sizes of the scale bars as labeled.