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. 2024 Feb 5;20(2):e1011152. doi: 10.1371/journal.pgen.1011152

Fig 6. The roles of CCZ1 in Yl recycling and yolk granule biogenesis.

Fig 6

Confocal fluorescent microscopy and DIC imaging of stage 10 egg chambers with oocyte-specific expression of dsRNA against CCZ1from flies (A-B4) carrying genome-tagging Yl-eGFP-3xHA reporter, co-labeled with phalloidin (red) and antibodies against endogenous Rab7 (green) and GFP for Yl (blue), or (C, D) with ectopic expression of 2xFYVE-GFP, co-labeled with antibody against GFP (green) and phalloidin (red), presented as overlaying images in color or as individual channels in gray, as annotated. (B-B4) and (D) are high-magnification views of the cortex regions highlighted in (A) and (C), respectively. Genotypes: The samples were from adult females flies heterozygous for both matalpha4-GAL-VP16 (BDSC #7062) and the following PI(3)P or Yl and UAS-transgenic RNAi lines: (A,B) P{TRiP.HMJ24129}attP40 (#62889); p{mini-W+, yl-eGFP-3xHA}. (C,D) P{w(+mC) = UAS-GFP-myc-2xFYVE}2 (#42172), P{TRiP.HMJ24129}attP40 (#62889). The sizes of the scale bars as annotated.