Table 1. Summary of phenotypes in oocytes overexpressing YFP-tagged Rab4, Rab5, Rab7 and Rab11 proteins.

UASp-YFP-Rab Lines	BDSC stock #	Phenotypes of stage 10 oocytes overexpressing YFP-Rab proteins driven by matalpha4-GAL-VP16 driver
		Subcellular localization of YFP-Rab protein	Rab7	Granule Size	F-Actin	Lysotracker
Rab4-WT	9767	Co-localize with Rab7-positive granules, also diffusive in cytoplasm	Normal	Normal	Normal	Normal
Rab4-CA	23268	Diffusive, with enrichment near oocyte cortex	Normal	Normal	Normal	Normal
Rab4-DN	9768	Diffusive, with enrichment near oocyte cortex	Normal	Normal	Normal	Normal
Rab5-WT	24616	Strongly enriched as puncta at cortex	Normal	Normal	Normal	Normal
Rab5-CA	9774	Enriched in puncta and on the membrane surface of large granules. Reduced enrichment at oocyte cortex.	Enriched in puncta and on the membrane surface of large granules. Reduced enrichment at oocyte cortex.	Very large	Normal near the cortex, more smaller F-actin puncta associated with large YFP-Rab5-CA granules inside the oocytes	Large granules are lysotracker-positive.
Rab5-DN	9778	Diffusive, with enrichment near oocyte cortex	Enrichment at oocyte cortex. More diffusive Rab7 in the cytoplasm. Few large Rab7-positive granules exist	Small granules	Normal	Existence of few very small lysotracker-positive granules near the posterior of oocytes
Rab7-WT	23641	Enriched at oocyte cortex and on the surface of Rab7-positive granules	Normal	Normal	Normal	Normal
Rab7-CA	24103	Enriched at oocyte cortex and on the surface of Rab7-positive granules	Normal	Normal	Normal	Normal
Rab7-DN	9778	Diffusive, with enrichment near cortex	Diffusive	Large	Aberrant "rings" on some Rab7-positive granules	Normal
Rab11-WT	50782	Enriched as small puncta at oocyte cortex.	Normal	Normal	Normal	Normal
Rab11-CA	23260	Diffusive, with enrichment near oocyte cortex	Normal	Normal	Normal	Normal
Rab11-DN	9792	Diffusive, with enrichment near oocyte cortex	Diffusive, reduced enrichment at oocyte cortex, increased levels near the posterior of the oocytes	Few visible and very small-sized granules	Aberrant small F-actin aggregates inside the oocytes	Existence of few small lysotracker-positive granules near the posterior of oocytes