Figure 2. No effect of DAP on endocytosis.
In the absence of bafilomycin, there are no significant differences in post-stimulus endocytosis rates between WT VAMP2 and any of the variants, or with DAP treatment. Data are means ± SEM of the change in fluorescence (ΔF) normalized to initial fluorescence (F0) over at least 22 boutons per coverslip from 7 to 9 coverslips and at least two independent cultures. Significance determined by t-tests.