TABLE 1.
Effect of RB C-terminal fragments on cell cycle progression
| Transfected RB plasmid | Nocadozolea | Cell cycle profileb
|
||
|---|---|---|---|---|
| %G1 | %S | %G2/M | ||
| pCMV | − | 41.0 | 33.9 | 25.1 |
| pCMV-wtRB | − | 69.7 | 12.1 | 18.1 |
| + | 68.0 | 12.8 | 19.1 | |
| pCMV-SE | − | 63.9 | 27.3 | 8.8 |
| + | 61.6 | 20.7 | 17.6 | |
| pCMV-SEΔ | − | 59.5 | 21.2 | 19.4 |
| + | 60.6 | 12.4 | 27.0 | |
| pCMV-SE-13S | − | 44.6 | 29.0 | 26.4 |
| + | 24.5 | 29.2 | 46.3 | |
a
+ indicates treatment with 0.1 μg of nocadozole per ml for 24 h as described in Materials and Methods.
b
DNA content of transfected Saos-2 cells was determined as described in Materials and Methods. Data presented are representative of results from three independent experiments. %G1, %S, and %G2/M, percentages of cells in G1, S, and G2/M, respectively.