Skip to main content
. 2023 Oct 27;120(1):56–68. doi: 10.1093/cvr/cvad166

Figure 6.

Figure 6

RBPMS directly modulates exon splicing of target genes. (A–C) Schematic of the Ttn exon 11 (EX11), Ttn exon 47 (EX47), and Pdlim5 exon 5 and 6 (EX5, EX6) minigene reporter constructs. Minigene reporter constructs were transfected alone or with low (+), high (++)-dose Rbpms-expressing vector in HEK293 cells. Exon inclusion events were evaluated by RT–PCR analysis. (D) Schematic of RBPMS and RBPMS-F65A/K100E mutant protein structures. Western blot analysis showing RBPMS and mutant protein expression in HEK293 cells. (E, F) RT–PCR analysis showing minigene reporter splicing results of Ttn-EX11 and Ttn-EX47 by wildtype (WT) or F65A/K100E mutant RBPMS. (G, H) Schematic of the minigene reporter constructs containing wildtype and intronic CAC-motif mutated Ttn exon 11 (EX11) and Ttn exon 47 (EX47). Minigene reporter constructs were transfected alone or with Rbpms-expression vector in HEK293 cells. Exon inclusion event was evaluated by RT–PCR analysis.