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. 2023 Oct 25;120(1):82–94. doi: 10.1093/cvr/cvad158

Figure 7.

Figure 7

TNF-α triggers activation-induced cell death of heart-reactive CD4+ T cells. TCRM splenocytes were pulsed with α-MyHC peptide in the absence (control) or presence of 5 ng/mL TNF-α (+TNF) or 10 µg/mL anti-TNF-α neutralizing antibody (+anti-TNF) for 7 days. (A–D) Quantification of live (A), apoptotic (B), late apoptotic (C), and necrotic (D) CD4+ T cells at Day 7 (n = 6). (E) Comparison of live and necrotic CD4+ T cells of control groups. P values calculated with one-way ANOVA followed by multiple comparison using Fisher’s LSD test. ns P > 0.05, *P < 0.05, **P < 0.01, ***P < 0.001.