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. 2024 Jan 24;4(2):100290. doi: 10.1016/j.bpsgos.2024.100290

Figure 6.

Figure 6

Pharmacological treatment of EGTA-AM and not bicuculline eliminates RSBs and rescues network bursting phenotype. (A) Representative raster plot of WIBR3 WT and null networks before and after bicuculline treatment. WIBR3 WT networks did not reverberate (NR-WT) whereas WIBR3 null networks did (R-NULL). (B–E) Quantification of (B) number of minibursts per RSB, (C) network event duration, (D) network event frequency, and (E) miniburst frequency in pre- and postbicuculline-treated WIBR3 networks. (F) Representative raster plot of WIBR3 NR-WT and R-NULL networks before and after EGTA-AM treatment. (G–I) Quantification of (G) number of minibursts per RSB, (H) network event duration, and (I) network event frequency in pre- and post-EGTA-AM-treated WIBR3 networks. (J) Representative raster plot of WIBR3 NR-WT networks before and after DMSO treatment. (K) Representative raster plot of WIBR3 R-NULL networks before and after EGTA treatment. (L) Representative raster plot of WIBR3 R-NULL networks before and after DMSO treatment. (M–Q) Quantification of (M) number of minibursts per RSB, (N) network event frequency, (O) network event duration, (P) mean interspike interval within network event, and (Q) median/mean ISI within network event in pre- and post-DMSO-treated WT networks (DMSO-WT), pre- and post-EGTA-AM-treated null (EGTA-NULL) networks, and pre- and post-DMSO-treated null (DMSO-NULL) networks. Point plots use mean (B–E, G–I) and median (M–Q) ± 95% CIs. Fold change label indicated over respective line plot. Statistical significance was evaluated using Mann–Whitney test, independent samples Student’s t test, Kruskal–Wallis and Dunn’s post hoc test. ∗p < .05, ∗∗p < .01, ∗∗∗p < .001. ISI, interspike interval; NE, network event; ns, not significant; RSB, reverberating super burst; WT, wild-type.