Extended Data Fig. 2. DCPs efficiently generate cDCs in distinct organs of recipient mice.
a, Engraftment of CD45.1+ cells derived from DCPs, moDCs and cDC1-like cells (mean ± SEM; n = 5 mice for PBS and n = 8 for DCPs, moDCs and cDC1-like cells) in spleen (shown as relative frequency, left, and absolute cell counts, right), BM (mean ± SEM; n = 8 mice), lung (mean ± SEM; n = 5 mice for PBS and n = 8 for DCPs, moDCs and cDC1-like cells) and liver (mean ± SEM; n = 5 mice for PBS, n = 6 for moDCs and cDC1-like cells, and n = 8 for DCPs) of MC38 tumor-bearing mice, 4 days after the last cell dose. Statistical analysis by one-way ANOVA with Tukey’s multiple comparison test. b, Donor cell chimerism in cDC1 and cDC2 of MC38 tumors after DCP transfer in an independent experiment (mean ± SEM; n = 7 mice). c, Donor cell chimerism in cDC1, cDC2, cDCs, macrophages, or Kupffer cells of spleen (mean ± SEM; n = 5 mice for PBS and n = 8 for DCPs, moDCs and cDC1-like cells), lung (mean ± SEM; n = 5 mice for PBS and n = 8 for DCPs, moDCs and cDC1-like cells) and liver (mean ± SEM; n = 5 mice for PBS, n = 6 for moDCs and cDC1-like cells, and n = 8 for DCPs). Note that liver-derived cells display some autofluorescence, which gives background signal in the CD45.1 channel. Statistical analysis by one-way ANOVA with Tukey’s multiple comparison test. Each data point represents one sample from an independent mouse.