Extended Data Fig. 1. DCPs efficiently generate cDCs in recipient mice.
a, Left: Phenotype of DCPs before enrichment of lineage-negative cells. Right: Proportion of DCPs before and after enrichment (paired samples) in several independent experiments (n = 6). b, Flow cytometry analysis of enriched DCPs from a representative experiment (gated on live cells). c, Flow cytometry dot plots showing the phenotype of cDC1-like cells, moDCs, and enriched DCPs, in one representative experiment. d, Flow cytometry dot plots showing the phenotype of enriched DCPs from representative wild-type (WT; top panels) or Batf3–/– (bottom panels) mice. e, Left: Engraftment of CD45.2+ cells derived from WT or Batf3–/– DCPs in the spleen of representative CD45.1 recipient mice. Right: Quantification of donor-derived cells in the spleen of recipient mice (mean ± SEM; n = 2 mice for PBS and n = 5 for WT and Batf3–/– DCPs). Statistical analysis by one-way ANOVA with Tukey’s multiple comparison test. Each data point represents one sample from an independent cell culture or mouse.