Figure 2.

NuS treatment accelerates neuron-like development and proliferation in SH-SY5Y cells. (A) EVOS microscopy images of undifferentiated SH-SY5Y cells in SF group, FBS group, and NuS group on day 2, day 4, and day 6. Scale bar = 200 μm. (B) Cell proliferation quantitation (n = 8 biological replicates). Cell proliferation assays were conducted from day 1 through day 6 of the culture period. Data are analyzed with Two-way ANOVA followed by Tukey’s multiple comparison test and paired t-test and are represented as mean ± SEM. #p ≤ 0.05, ##p ≤ 0.01, *p ≤ 0.05, **p ≤ 0.01.