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. 2024 Feb 27;14:4775. doi: 10.1038/s41598-024-55516-5

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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NuS treatment accelerates neuron-like morphology development in cells. Immunofluorescent images for MAP2 (A) and NF-L (B) of SH-SY5Y cells in FBS group and NuS group on day 2, day 4, and day 6. Nuclei in blue, MAP2 in green, NF-L in magenta. Scale bar = 100 μm. Bar graphs are representing the IF intensity quantification reflecting the MAP2/Hoechst (A) and NF-L/Hoechst (B) intensity, n = 9 areas. Data are analyzed with Unpaired t-test and are represented as mean ± SEM. *p ≤ 0.05, **p ≤ 0.01.

NuS treatment accelerates neuron-like morphology development in cells. Immunofluorescent images for MAP2 (A) and NF-L (B) of SH-SY5Y cells in FBS group and NuS group on day 2, day 4, and day 6. Nuclei in blue, MAP2 in green, NF-L in magenta. Scale bar = 100 μm. Bar graphs are representing the IF intensity quantification reflecting the MAP2/Hoechst (A) and NF-L/Hoechst (B) intensity, n = 9 areas. Data are analyzed with Unpaired t-test and are represented as mean ± SEM. *p ≤ 0.05, **p ≤ 0.01.