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. 2024 Feb 27;14:4775. doi: 10.1038/s41598-024-55516-5

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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SH-SY5Y cells can be differentiated using NuS supplement. (A) EVOS microscopy images of differentiating SH-SY5Y cells in FBS group and NuS group on day 1, day 4, day 6, day 9 and day 11. Scale bar = 400 μm. (B) EVOS microscopy images of undifferentiated and differentiated (on day 11) SH-SY5Y cells in FBS and NuS groups. Scale bar = 400 μm. (C) Immunofluorescent images for MAP2, NeuN and β3-Tubulin neuronal markers on undifferentiated and differentiated SH-SY5Y cells. Nuclei in blue, MAP2 in green, NeuN in light green, β3-Tubulin in violet. Scale bar = 100 μm. Bar graphs are representing the IF intensity quantification reflecting the MAP2/Hoechst, NeuN/Hoechst and TU20/Hoechst intensity, n = 6 areas. Data are analyzed with Unpaired t-test and are represented as mean ± SEM. **p ≤ 0.01, ****p ≤ 0.0001.