Fig. 1.

Postprandial NOx (A) and E-selectin (B) concentrations measured in the cell culture supernatant following 24-h incubation of human aortic endothelial cells with plasma samples isolated from a subset of RESET participants who were exposed to sequential high-fat mixed-meal challenges (breakfast at 0 min and lunch at 330 min) representative of the FA-modified and conventional (control) dairy diets following each 12-week dietary intervention. All experiments were performed using duplicate wells and concentrations were corrected for the protein content in each well. Values are untransformed and unadjusted means ± SEMs (n = 11). The dotted lines represent the timing of the second meal (330 min). Linear mixed-model analysis was used to explore the effects of treatment and time, with an adjustment made in all cases for fixed—(period, time, treatment, sex, age, and BMI) and random—(participant) effect covariates. P < 0.05 was deemed significant. FA fatty acid, NOx total nitric oxide