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. 2024 Feb 14;15:1361747. doi: 10.3389/fneur.2024.1361747

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Copyright © 2024 Wood, Nowak and Fuchs.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Analysis of autofluorescence – (Non-GCaMP Associated Fluorescence [NGAF]) in damaged cochlear epithelium; images from Th^2ACreEr x Gcamp6f^fl/fl mouse tissue of Nowak et al. (36). (A1) Standard deviation image of time series z-projection spanning 5.5 min in total (80 ms/slice). A central region of initial damage (“burn”), and a type II neuron are visible. (A2) A single 80 ms slice 14 s after the focused laser ablation (central bright green circle) shows one time point of spreading NGAF fluorescence. Red outline shows approximate initial response area (burn plus 9 s). Regions of interest (ROIs) from A1 overlaid. Colored asterisks denote exemplar ROIs used to determine wave velocity. (B) Average pixel intensity from Z projection for asterisked ROIs in A2.