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. 2023 Dec 18;52(4):1930–1952. doi: 10.1093/nar/gkad1155

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© The Author(s) 2023. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 7. — Constitutive nuclear localization of AGO2 (NLS-AGO2 knock-in) phenocopies overconfluent culture. (A) Western blot validation of AGO2 nuclear localization in CRISPR/Cas9-NLS-AGO2 knock-in HCT116 (NLS-AGO2). (B) Immunofluorescence validation of AGO2 nuclear localization in NLS-AGO2 compared to WT HCT116. (C–E) RT-qPCR results describing top candidate miRISC target expression changes in NLS-AGO2 cell line relative to WT (N = 3). Significance denoted by *’s with (C) IGF1R, P= 0.0004, (D) OLFML2A, P= 0.005, (E) NOTCH3, P= 0.03.