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. 2023 Dec 18;52(4):1930–1952. doi: 10.1093/nar/gkad1155

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© The Author(s) 2023. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 8. — Constitutive cytoplasmic localization of AGO2 sufficient to rescue repression. (A) Scheme showing transfection of WT-AGO2-GFP and NES-AGO2-GFP in AGO1/2/3–/– cells. (B, C) Expression and localization of WT-AGO2-GFP and NES-AGO2 evaluated by western or microscopy. (D–F) Expression of WT-AGO2-GFP and NES-AGO2 restores repressed expression of IGF1R, NOTCH3, and OLFML2A.