Table 1.

Catalog of validated triggers. Shown are predicted pairing architectures of miRNA binding sites in naturally occurring triggers validated by knockout and/or binding site mutagenesis studies; blue shading indicates seed binding, green shading indicates 3′ pairing, and red text denotes the dinucleotides adjacent to the seed binding region. ^‡These triggers regulate more than one miRNA within a family, but for simplicity, only one pairing diagram is shown. The type of seed binding site, the length in nts of 3′ Watson-Crick pairing and in parentheses 3′ pairing that includes G:U wobbles and one nt bulges, and the minimum free energy (MFE) in kcal/mol of 3′ pairing to nts 11 and higher in the miRNA, as determined by RNAstructure DuplexFold (103), are also provided, as well as the virus/species and reference in which validation occurred