Fig. 2.

Nervonic acid (NA) suppressed the replicative senescence of Wharton’s jelly-derived mesenchymal stem cells. (A) Experimental scheme for identification of senescence inhibition through NA after induction of replicative senescent mesenchymal stem cells (MSCs). (B) Cell viability was assessed after treatment with various NA concentrations. (C) β-Galactosidase staining (scale bar=250 μm) and (D) area measurement of cells was performed with or without NA. Data are presented as mean±SEM. The significance of the differences was assessed by one-way ANOVA (***p<0.001). NS-MSCs: non-senescent MSCs; RS-MSCs: replicative senescent MSCs; CCK-8: Cell Counting Kit-8; DMSO: dimethyl sulfoxide.