Fig. 3.

Anti-tumor activity of the MCD nanoparticles in vitro. (A) Cytotoxic effect for 143B and U2OS cells of the MD and MCD nanoparticles with or without irradiation. The unit of concentration is μg/L. (B) Live/Dead fluorescence images for 143B and U2OS cells in each group. Scale bar: 50 μm. (C) Quantified results of Live/Dead staining. (D) Colony formation experiment of cells stained with crystal violet. (E) Quantitative results of the colony formation experiment. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)