Fig. 6.

Mechanism of inhibitory effect of materials on osteoclasts. (A) Flow cytometry for ROS of cells with different treatments. BMMs were treated with or without 125 μg/mL MCD with 6 h for cytophagy and washed by PBS. final culture with osteoclastogenic induction medium for 2 h. (B) Expression of osteoclast marker genes, including c-Fos, CTSK, NFATc1, and DC-STAMP. (C, D) TRAP staining, number, and size of the TRAP⁺ osteoclasts with > 5 nuclei were quantified. (E) WB analyses of protein expressions in different groups. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001.