Scheme 2. Substrate Scope for the ADH-Catalyzed Desymmetrization of Prochiral Dialdehydes.

Reaction conditions: 1a (10 mM), NADP⁺ (1 mM), glucose (20 mM), GDH (2.5 mg mL^–1), ADHs (0.1 g mL^–1 of whole cell with 6 units mL^–1 of DNaseI and 1 mg mL^–1 of lysozyme), DMSO (5% v/v) in NaPi buffer (pH 7.4, 50 mM), at 30 °C, 3–24 h, 800 rpm. Total volume: 1 mL. *3b (5% by ADH-R2), 3c (3% by ADH-R1), 3i (3% by ADH-R7), 3k (16% by ADH-R1 and 53% by ADH-R2), 3l (2% by ADH-R1) were formed at the sampling time, and the diol products were not found for the rest of the substrates.