Table 1. Screening of ADHs Utilizing 1a as the Model Substratea.
| entry | enzyme | sources | types | 2a [%] | 3a [%] | ee [%] | configuration of 2a |
|---|---|---|---|---|---|---|---|
| 1 | ADH-R1 | Burkholderia gladioli | SDR | 94.3 ± 3.1 | 3.0 ± 0.8 | 80.4 ± 0.8 | S |
| 2 | ADH-R2 | Ralstonia sp. | SDR | 92.4 ± 2.5 | 1.9 ± 0.9 | 99.9 ± 0.1 | S |
| 3 | ADH-R3 | Lactobacillus kefiri | SDR | 47.7 ± 0.3 | 1.6 ± 0.3 | 70.1 ± 0.7 | S |
| 4 | ADH-R4 | Oenococcus alcoholitolerans | SDR | 87.5 ± 0.7 | 11.4 ± 1.8 | 99.4 ± 0.2 | S |
| 5 | ADH-R5 | Zymomonas mobilis | MDR | 87.9 ± 1.8 | 2.9 ± 0.4 | 88.3 ± 0.0 | R |
| 6 | ADH-R6 | Parageobacillus thermoglucosidasius | MDR | 90.6 ± 1.8 | 2.6 ± 0.4 | 92.5 ± 1.5 | R |
| 7 | ADH-R7 | Thermus thermophilus | SDR | 95.4 ± 0.3 | 3.7 ± 0.3 | 99.9 ± 0.1 | R |
| 8 | ADH-R8 | Rhodopseudomonas palustris | MDR | 93.5 ± 1.4 | 2.9 ± 0.6 | 95.5 ± 5.4 | R |
| 9 | ADH-R9 | Rhizobium etli | MDR | 96.7 ± 1.2 | 2.9 ± 1.0 | 99.4 ± 0.1 | R |
| 10 | ADH-R10 | Pseudomonas meliae | MDR | 95.5 ± 0.9 | 4.3 ± 0.9 | 99.9 ± 0.1 | R |
a
Reaction conditions: 1a (10 mM), NADP+ (1 mM), glucose (20 mM), glutamate dehydrogenase (GDH) (2.5 mg mL–1), ADHs (0.1 g mL–1 of whole cell with 6 units mL–1 of DNaseI and 1 mg mL–1 of lysozyme), DMSO (5% v/v) in NaPi buffer (pH 7.4, 50 mM), at 30 °C, 800 rpm. Total volume: 1 mL.