Figure 2.

NPY expression in the colon and plasma of mice subjected to FMT by receiving feces from healthy control and UC patients. (a) mRNA expression of NPY family genes (NPY, PPY, and PYY) and their receptors (NPY1R, NPY2R, PPYR1, and NPY5R) in the colon of mice subjected to FMT by receiving feces from healthy control (HC-FMT; n = 6) and UC patients (UC-FMT; n = 6). (b) Representative bands and graphs showing western blot analysis of NPY protein in the colon of HC-FMT and UC-FMT mice. (c) Plasma levels of NPY in NC, HC-FMT, and UC-FMT mice measured by an enzyme-linked immunoassay kit. (d and e) colon and brain tissues were collected from control mice that were not subjected to FMT challenge. The mRNA (d) and protein (e) expression levels of NPY and its receptor NPY1R were evaluated in the colon and brain of the mice. Statistical significance between two groups was calculated using two-tailed unpaired t-test (*p < 0.05).