Figure 6.

Effect of capsular polysaccharide (CP) of EM on the levels of NPY in mice and enteric endocrine cells. (a) Schematic diagram of the animal study. Capsular polysaccharide (CP) of EM (NK1516-CP or ATCC882-CP) (n = 6) or normal control (NC; sterile saline) (n = 6) was orally administrated once a day for 5 days. (b) Representative bands and graphs showing western blot analysis of NPY protein in the colon of mice orally administered with NK1516-CP. (c) Plasma levels of NPY in mice orally administered with NK1516-CP measured by an enzyme-linked immunoassay kit. (d) Representative bands and graphs showing western blot analysis of NPY protein in the hippocampus of mice orally administered with NK1516-CP (e) Representative bands and graphs showing western blot analysis of NPY protein in the hippocampus of mice orally administered with ATCC882-CP or NK1516-CP. (f and g) enteric endocrine cells STC1 were treated with cytosol or CP fraction of EM (NK1516) for 24 h. (f) mRNA expression of NPY in STC cells measured by qRT-PCR. (g) Representative bands and graphs showing western blot analysis of NPY protein in STC cells. Statistical significance between two groups was calculated using two-tailed unpaired t-test (*p < 0.05).