FIG. 3.

Quantitative analysis of H₂O₂ production by neutrophils during the oxidative burst following IgG or TPA stimulation. Primary bone marrow neutrophils were derived from either wild-type or Syk^−/− bone marrow chimeras. Cells were incubated with IgG-opsonized zymosan particles, and H₂O₂ production was determined by using horseradish peroxidase-catalyzed oxidation of scopoletin. The ability to generate a response to an FcγR-independent signal was tested by exposure of an equivalent cell sample to 0.1 μM TPA. Each bar represents the mean and error range of two independent experiments.