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Cell viability of macrophages and activated macrophages were validated in vitro. (A) Cytotoxicity assay of macrophages with different concentrations of HF (0, 2, or 4 nM) for 24 h (n = 3 per group). (B) Effect of different concentrations of HF (0, 2, or 4 nM) on the viability of activated macrophages after co-incubation for 24 h (n = 3 per group). The data are represented as the means ± SD; * p < 0.05, ** p < 0.01
