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. 2024 Feb 27;55:25. doi: 10.1186/s13567-024-01266-1

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Expression of MUC2 and DCLK1 in enteroid 2D monolayers and 3D enteroids stimulated with eqIL-4/IL-13. Relative gene expression of A MUC2 and B DCLK1 transcripts in transwell-grown equine enteroid monolayers basolaterally stimulated with eqIL-4/IL-13 and compared to untreated controls. The gene expression was normalized to the geometric mean for the reference genes HPRT, SDHA and GAPDH and compared to the mean ΔΔCt of the untreated controls. The monolayers were generated from two individual horses and used in two (horse 1) and three (horse 2) separate experiments, giving a sample size of N = 5. C, D Confocal images of equine 3D enteroids cultured in plain growth medium or stimulated with eqIL-4/IL-13 for 48 h before staining with DAPI (blue) and C MUC2 (green) or D DCLK1 (red). Representative images from one horse. E Proportion of cells positive for DCLK1 staining. Mean ± SD of 4 technical replicates. Scale bars = 100 µm for C and 50 µm for D. *P < 0.05, **P < 0.01.