Fig. 4.

Knocking out SLC7A2 in STHdhQ111 cells attenuates neuroinflammatory responses. A Genotyping of SLC7A2KO clones shows a large fragment deletion in SLC7A2 PCR products compared to that in parental STHdhQ111 cells. B Intracellular NO production in real time in WT and SLC7A2KO cells in response to IFNγ/LPS treatment. C Quantification of intracellular NO levels 24 h after IFNγ/LPS treatment under different conditions as indicated. ****p < 0.0001. D Quantification of medium NO levels 24 h after IFNγ/LPS treatment under different conditions as indicated. **p = 0.0032, ****p < 0.0001. In C, D, two-way ANOVA followed by Tukey’s multiple comparisons test. E Western blot analysis of iNOS expression under different conditions as indicated. β-Actin was used as a loading control. The bottom panel shows the quantification of iNOS expression from three independent Western blots by densitometry analysis. *p = 0.016, ^#p = 0.022, one-way ANOVA followed by Tukey’s multiple comparisons test