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Activation of Plx1 in Cdc25C-injected oocytes. Oocytes were microinjected with either wild-type Cdc25C or phosphatase-inactive Cdc25C(C457A) (25 ng per oocyte), and groups of six oocytes were frozen at the indicated times. Extracts were prepared, and histone H1 kinase and Plx1 activities were determined. Symbols for histone H1 kinase activity: ▪, Cdc25C(C457A); •, Cdc25C. Symbols for Plx1 activity: □, Cdc25C(C457A); ○, Cdc25C.
