FIGURE 6.

Cell death upon INHBA knockdown. A, Cell death of native UDSCC1, Mock KD, INHBA KD1/KD2 and native UDSCC2 cells as assessed by Annexin V/7-AAD staining and flow cytometry. Results are shown as mean ± SD of the percentages of dead cells normalized to UDSCC1 (n = 3). Conditions were compared with native UDSCC1 using unpaired t test with *, P ≤ 0.05; **, P ≤ 0.01; ****, P ≤ 0.0001; and ns, P > 0.05. B, Representative flow cytometry plots for each condition. Gates show early apoptotic in the lower right quadrant, late apoptotic in the upper right quadrant and necrotic cell populations in the upper left quadrant. t0-plots represent the baseline timepoint right before transfection.