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. 2023 Sep 25;40(3):237–245. doi: 10.5511/plantbiotechnology.23.0717a

Figure 2. Summary of the mutant sequences of the genome editing target site in TaQsd1. Sequencing chromatograms of subgenome-specific TaQsd1 DNA fragments surrounding the sgRNA target site. DNA samples were obtained from T1 seedlings from T0 plants carrying a mutation in all six copies of TaQsd1. Solid lines indicate sequences corresponding to the sgRNA sequence. Triangles indicate the positions of deletions. Dotted lines indicate the positions of the PAM sequence.

Figure 2. Summary of the mutant sequences of the genome editing target site in TaQsd1. Sequencing chromatograms of subgenome-specific TaQsd1 DNA fragments surrounding the sgRNA target site. DNA samples were obtained from T1 seedlings from T0 plants carrying a mutation in all six copies of TaQsd1. Solid lines indicate sequences corresponding to the sgRNA sequence. Triangles indicate the positions of deletions. Dotted lines indicate the positions of the PAM sequence.