Table 2. Summary of the transformation experiments, number of transgenic plants, and mutations of TaQsd1.

Heat treatment	sgRNA promoter	Number of experiments	Number of embryos	Transgenic plants (number, rate)*	Genome-edited plants (number, rate)	Mutant genotype** and plant line number
“25°C” (25°C, 5 day)	OsU6	8	567	12, 2.12%	0, 0%
	TaU6	8	586	13, 2.22%	5, 0.85%	AABBDd, T7 AABbDD, T18 AaBbDd, T19 aabbdd, T12, T26
“30-1d” (30°C, 1 day)	OsU6	8	603	14, 2.32%	1, 0.17%	AABbDD, O20
	TaU6	8	585	13, 2.22%	6, 1.03%	AABbDd, T15 AaBbdd, T2 aabbdd, T10, T16, T23, T24

*The rate was calculated based on the number of regenerated PCR-positive transformants per callus piece. Only one regenerated plant per callus piece was retained. The Cas9 or bar DNA fragment was detected by PCR. **TaQsd1 genotype.