Table 3. Transgene integration sites, as estimated based on the segregation ratios in the progenies.
| Condition | Number of mutations in T0 plants | Segregation of the transgene in T1 plants (+ : −) | Segregation of the transgene in F1 plants (+ : −) | Estimated number of transgene integration sites | Plant line number |
|---|---|---|---|---|---|
| OsU6, “25°C” | 0 | 9 : 7–13 : 3 | NA | 1 | O3–O6, O9, O14, O21 |
| 14 : 2 | NA | 1–2 | O11, O15 | ||
| 15 : 1 | NA | 2 | O25 | ||
| 16 : 0 | NA | >2 | O10, O26 | ||
| OsU6, “30-1d” | 0 | 11 : 5–13 : 3 | NA | 1 | O8, O18, O19 |
| 14 : 2 | NA | 1–2 | O12, O17 | ||
| 16 : 0 | NA | >2 | O1, O2, O7, O13, O16, O22–O24 | ||
| 1 | 13 : 3 | NA | 1 | O20 | |
| TaU6, “25°C” | 0 | 12 : 4–13 : 3 | NA | 1 | T3, T21 |
| 14 : 2 | NA | 1–2 | T17, T25 | ||
| 15 : 1 | NA | 2 | T5 | ||
| 16 : 0 | NA | >2 | T6, T8, T20 | ||
| 1 | 13 : 3 | NA | 1 | T7 | |
| 15 : 1 | NA | 2 | T18 | ||
| 3 | 12 : 4 | NA | 1 | T19 | |
| 6 | 16 : 0 | NA | >2 | T12, T26 | |
| TaU6, “30-1d” | 0 | 11 : 5–13 : 3 | NA | 1 | T4, T11, T22 |
| 14 : 2 | NA | 1–2 | T9, T13 | ||
| 16 : 0 | NA | >2 | T1, T14 | ||
| 2 | 16 : 0 | NA | >2 | T15 | |
| 4 | 16 : 0 | NA | >2 | T2 | |
| 6 | 16 : 0 | 21 : 3 | 3 | T24 | |
| 16 : 0 | 24 : 0 | >4 | T16, T23 | ||
| no seeds | NA | T10 |
NA, not applicable.