Table 1. AG1 target sequences determined by Sanger sequencing analysis.

Line	Amplified band	Allele	Target 1	In/del	Target 2	In/del
WT	~300 bp	1	TCGTCGAGGAAATCAGGCAGAGG	WT	ACTTTCTGCAAGCGCAGAAGTGG	WT
		2	TCGTCGAGGAAATCAGGCAGAGG	WT	ACTTTCTGCAAGCGCAGAAGTGG	WT
No. 1	~300 bp	1	TCGTCGAGGAAATCAGGGCAGAGG	+1 bp	ACTTTCTGCA-------AAGTGG	−7 bp
		2	TCGTCGAGGAAATCAGGACAGAGG	+1 bp	ACTTTCTGCAAG-----AAGTGG	−5 bp
No. 4	~300 bp,	1	TCGTCGAGGAAATCAGGGCAGAGG	+1 bp	ACTTTCTGCAA----AGAAGTGG	−4 bp
	~450 bp	2	TCGTCGAGGAAATCAGGACAGAGG	+1 bp	ACTTTCTGCAAGCG(−12+166)	+154 bp
No. 5	Same pattern to No. 4	1	Not determined		Not determined
		2	Not determined		Not determined
No. 6	~300 bp	1	TCGTCGAGGAAATCAGGCAGAGG	WT	ACTTTCTGCAAG-----AAGTGG	−5 bp
		2	TCGTCGAGGAAATCA--CAGAGG	−2 bp	ACTTTCTGCAAGCGC-GAAGTGG	−1 bp
No. 8	~300 bp,	1	TCGTCGAGGAAA---------GG	−9 bp	ACTTTCTGC--------AAGTGG	−8 bp
	~350 bp	2	TCGTCGAGGAAATCAGG-AGAGG	−1 bp	A(+51)	+51 bp
No. 9	~300 bp	1	TCGTCGAGGAAATCAGGCAGAGG	WT	ACTTTCTGCAAGCGC--AAGTGG	−2 bp
		2	TCGTCGAGGAAATCAGGCAGAGG	WT	ACTTTCTGC--------AAGTGG	−8 bp
No. 12	~300 bp	1	TCGTCGAGGAAATCAGGCAGAGG	WT	AC------------------TGG	−18 bp
		1	TCGTCGAGGAAATCAGGCAGAGG	WT	AC---------------------	−23 bp
		2	TCGTCGAGGAAATCAGGACAGAGG	+1 bp	ACTTTCTGC--------AAGTGG	−8 bp
No. 13	~300 bp	1	TCGTCGAGGAAATCAGGACAGAGG	+1 bp	ACTTTCT----------AAGTGG	−10 bp
		2	TCGTCGAGGAAATCAGGTCAGAGG	+1 bp	ACTT----------------TGG	−16 bp
No. 14	~300 bp	1	TCGTCGAGGAAATCAGGGCAGAGG	+1 bp	ACTTTCTGCAAGCGC--AAGTGG	−2 bp
		2	TCGTCGAGGAAATCA--CAGAGG	−2 bp	ACTTTCTGCAAGCGCAGGAAGTGG	+1 bp