Table 2. Off-target analysis of homologous sequences in AG2 determined by Sanger sequencing analysis.

Line	Allele	Target 1 homologous sequence	In/del	Target 2 homologous sequence	In/del
WT	1	AATTCTAGGAAAAGTGGTAGAGG	WT	ACTTTCTGCAAACGCAGAAGTGG	WT
	2	AATTCTAGGAAAAGTGGTAGAGG	WT	ACTTTCTGCAAACGCAGAAGTGG	WT
No. 6	1	AATTCTAGGAAAAGTGGTAGAGG	WT	ACTTTCTGCAAACGCAGAAGTGG	WT
	2	AATTCTAGGAAAAGTGGTAGAGG	WT	ACTTTCTGCAAA-----AAGTGG	−5 bp