Table 1. Estimated allele numbers of mutants in the GBSS1 gene in the transformants.

Number of mutant alleles	Number of gRNAs
	With dMac3		Without dMac3
	Two	Three	Two	Three
0	6	6	7	6
1	7	5	3	3
2	8	4	0	5
3	5	6	0	6
4	2	8	0	1
Total	28	29	10	21

The number of mutant alleles was estimated by the CAPS analysis of the mutant lines. Mutant lines detected by the genome editing using two and three gRNAs in the CRISPR/dMac3-Cas9 (left) and conventional CRISPR/Cas9 (right) vectors are shown.