Table 1. Shoots and genome editing lines obtained for each regeneration-promoting gene in this experiment.
| Cultivar | Regeneration-promoting gene | Explants | Regenerated shoots* | HMA positive No transgene | Complete disruptant | Efficiency (%/ Regeneration) |
|---|---|---|---|---|---|---|
| Sassy | — | >200 | 0 | — | — | — |
| ipt | 427 | 224 | 2 | 1 | 0.9 | |
| BBM | 648 | 95 | 1 | 0 | 1.1 | |
| LEC2 | 440 | 91 | 0 | 0 | — | |
| ESR2 | 228 | 88 | 0 | 0 | — | |
| ESR1 | 478 | 98 | 1 | 0 | 1.0 | |
| Sayaka | — | >200 | 0 | — | — | — |
| ipt | 242 | 195 | 5 | 2 | 2.6 | |
| BBM | 266 | 198 | 0 | 0 | — | |
| ESR1 | 225 | 115 | 0 | 0 | — | |
| WUS | 219 | 56 | 0 | 0 | — | |
| May Queen | — | >200 | 0 | — | — | — |
| ipt | 820 | 282 | 0 | 0 | — | |
| BBM | 250 | 137 | 0 | 0 | — | |
| ESR1 | 608 | 302 | 0 | 0 | — | |
| WUS | 96 | 13 | 0 | 0 | — |
* Not include the number of non-normal/multiple shoots or tissues.