Table 2.

Distribution of copy numbers of Rcr1 and disease severity index (DSI) in the T₀–T₂ generations of canola lines transformed with the different vectors

Vector	T0	T1 of T0 with single-copy insert					T2 of T1 with two copies
	1 copy	2 copies	1 copy	0 copy	Total no. plants	DSI (%)	2 copies	1 copy	0 copy	Total no. plants	DSI (%)
pHHIGS-AP1	9	15	32	13	60	21.7 ± 5.8b	59	0	0	59	0 ± 0a
pHHIGS-CLV3	7	14	30	14	58	24.1 ± 1.6b	60	0	0	60	0 ± 0a
pHHIGS-Hsp18.2	10	14	33	13	60	21.7 ± 2.9b	60	0	0	60	0 ± 0a
DH12075	–	0	0	60	60	100 ± 0c	0	0	60	60	100 ± 0c

Copy number was determined by ddPCR; DSI was determined through pathogenicity tests. Different letters indicate significant differences in DSI within the T₁ and T₂ generations, as determined using ANOVA and post-hoc Tukey’s HSD test.