Figure 4.

Augmentation of the proportion of cells with cilia by CEP72 depletion. (A) The CEP72-depleted RPE1 cells were cultured in a serum-deprived medium for 48 h and immunoblotted with antibodies specific to CEP72 and α-tubulin. (B) CEP72 was depleted in the CEP250 and CROCC KO cells and subjected to coimmunostaining analysis with antibodies specific to PCM1 (cyan) and acetylated tubulin (magenta). Scale bar, 10 μm; inset scale bar, 2 μm. (C) Intensities of PCM1 at the basal bodies were determined. Within each box, the black center line represents the median value, the black box contains the interquartile range, and the black whiskers extend to the 10th and 90th percentiles. (D) The number of cells with cilia was counted. Graph values are expressed as mean and SEM. (C and D) More than 30 cells per group were counted in three independent experiments. Statistical significance was determined using one-way ANOVA with Tukey’s post hoc test (***, P < 0.001; n.s., not significant). Source data are available for this figure: SourceData F4.