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. 2024 Feb 28;223(4):e202105065. doi: 10.1083/jcb.202105065

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© 2024 Ryu et al.

This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).

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Figure 8. — Definition of the PCM1 domains for centrosome accumulation and cilia formation. (A) The PCM1 KO RPE1 cells were cultured in a serum-deprived medium for 48 h and coimmunostained with antibodies specific to PCM1 (cyan) and acetylated tubulin (magenta). (B) The number of cells with cilia was counted. (C) Schematic of the truncated mutants of FLAG-PCM1. Centrosome localizations of the PCM1 truncated mutants are summarized on the right. (D) Truncated mutants of FLAG-PCM1 were expressed in the PCM1 KO RPE1 cells and subjected to immunoblot analyses with antibodies specific to FLAG and GAPDH. (E) The cells were coimmunostained with antibodies specific to FLAG (cyan) and γ-tubulin (magenta). (F) The number of cells with cilia was counted. (A and E) Scale bars, 10 μm; inset scale bars, 2 μm. (B and F) More than 30 cells per group were counted in three independent experiments. Graph values are expressed as mean and SEM. Statistical significance was determined using one-way ANOVA with Tukey’s post hoc test (***, P < 0.001; n.s., not significant). Source data are available for this figure: SourceData F8.