Video 1.
Tumor-specific CD8+ T cells interact with DCs in the draining lymph node, related to Fig. 2. CD11c-eYFP mice were injected s.c. with MC38-OVA tumor cells. After 10 days, mice were adoptively transferred with naïve GFP-expressing OT-I CD8+ T cells and treated or not with anti-PD-1 mAb (250 µg, i.v.). Two-photon imaging of tumor-draining lymph nodes was performed on day 2. The movie shows contacts between OT-I CD8+ T cells (pseudocolored in magenta) and CD11c reporter positive cells (pseudocolored in cyan) in tumor-bearing mice but not in tumor-free mouse. Total duration = 29 min.