Fig 4. SCARB2 is not involved in EV-A71 binding to Jurkat cells, but necessary for viral replication.

PSGL-1 or SCARB2 were knocked out by CRISPR/Cas9 in Jurkat cells, and two each of clones were established. (A) Cells were infected with EV-A71 at 1 CCID₅₀ per cell on ice for 1 h, then washed (0 h), cultured, and harvested at 3 days (3 d) post-infection. (B) Cells were infected with EV-A71-EGFP at 10 CCID₅₀ per cell and cultured for 18 h. Infected cells were identified by flow cytometry to detect EGFP expression. Results are indicated as the mean and s.e. for triplicate samples.