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. Author manuscript; available in PMC: 2024 Feb 28.
Published in final edited form as: J Virol Methods. 2020 Oct 21;287:114004. doi: 10.1016/j.jviromet.2020.114004

Fig. 3.

Fig. 3.

RNA and antigen stability after gamma irradiation. rRT-PCR was performed on gamma irradiated samples with different doses described on X-axis (A). Only samples that demonstrated complete inactivation were assessed for RNA. All samples were run in triplicate and mean Ct value is represented with error bars on Y-axis. Ct value below 35 (cut-off line) is considered positive for ZIKV. For mock sample the value above 38 “may represent erratical amplification curves and is therefore considered negative” (Santiago et al., 2018). No template control (NTC) was undetermined. Ordinary one-way ANOVA gave P value <0.0001, R square = 0.9989. MAC-ELISA performed was performed on gamma irradiated samples with different doses described on X-axis (B). Samples that demonstrated complete inactivation were assessed by MAC-ELISA. All samples were run in triplicate and ZIKV specific antigen activity is represented with error bars on Y axis as O.D. (450 nm). Ordinary one-way ANOVA gave P value <0.0001, R square = 0.9548. Inactivation of each sample compared with live virus analyzed by unpaired t-test, 2 tailed column analysis. p value < 0.01 (**); < 0.001 (***); <0.0001 (****).