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. Author manuscript; available in PMC: 2024 Feb 28.
Published in final edited form as: J Virol Methods. 2020 Oct 21;287:114004. doi: 10.1016/j.jviromet.2020.114004

Table 1A.

Heat inactivation at 56 °C.

Temp Tube Volume Time Pfu/mL Inactivated (Y/N)
56 °C 2 mL 1 mL 1.5 h 1 × 105 N
1 × 106 N
1 × 107 N
2 h 1 × 105 N
1 × 106 N
1 × 107 N
2.5 h 1 × 105 N
1 × 106 N
1 × 107 N
56 °C 15 mL 10 mL 1.5 h 1 × 105 N
1 × 106 N
1 × 107 N
2 h 1 × 105 Y
1 × 106 N
1 × 107 N
2.5 h 1 × 105 Y
1 × 106 Y
1 × 107 Y
56 °C 50 mL 40 mL 1.5 h 1 × 105 N
1 × 106 N
1 × 107 N
2 h 1 × 105 Y
1 × 106 N
1 × 107 N
2.5 h 1 × 105 Y
1 × 106 Y
1 × 107 Y

A and 1B The inactivation of ZIKV by heat treatment was performed at 56 °C (Table 1A) and 60 °C (Table 1B). For heat treatment,10 mL of sample was dispensed into 15 mL conical tubes, 40 mL sample into 50 mL conical tubes and 1 mL sample into 2 mL screw cap tubes. For each condition, three titers of viral supernatant were tested: 1 × 105 PFU/mL, 1 × 106 PFU/mL and 1 × 107 PFU/mL. The tubes at 56 °C (Table 1A), were treated for 1.5–2.5 hours in 30 min increments. The tubes at 60 °C (Table 1B) were dispensed similarly and treated for 10 min and 1 h. Y: inactivation was successful (no CPE and no plaques); N: inactivation failed (positive for CPE or plaque formation).